Protein Method Blocking Buffers
SCANTIBODIES LABORATORY INC Heterophilic Blocking Tube
This item must be ordered in multiples of 50 per supplier requirements
Tube, Blocking; Heterophilic; Unique blocking reagent composed of specific binders which inactivate heterophilic antibodies; Allows rapid and simple elimination of false positive heterophilic interference in plasma for sandwich immunoassays.
Sigma Aldrich Fine Chemicals Biosciences Gelatin blocking buffer for Western blotting, powder blend | 1L
Gelatin blocking buffer for Western blotting, powder blend | 1L
LI COR BIOTECH LLC Intercept (PBS) Protein-Free Blocking Buffer (500 mL)
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited. They are an excellent choice for biotin-streptavidin detection and suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Cell Biolabs Inc PhosphoBLOCKER™ Blocking Reagent, 200 g dry blend (4 Liters prepared)
The utilization of anti-phosphoprotein antibodies in western blotting has become a commonly used tool for signal transduction research. Unfortunately, low levels of endogenous phosphoprotein in various cell lysates often can not be detected, even with high concentrations of antibody and long exposure times. Most commercially available western blot blockers (e.g. dry milk, serum) are sufficient to block the unreacted sites on the membrane, reducing the amount of nonspecific antibody binding during the assay; however, they are not designed to preserve phosphoprotein antigens during blotting. Cell Biolabs’ PhosphoBLOCKER™ contains a proprietary formulation that provides several advantages over conventional blockers: (1) It is designed specifically for phosphoprotein blotting; (2) It enhances low level phosphoprotein signal without increasing background; and (3) it is an easy to use premixed dry blend. 200 grams (reconstituted to 4 liters).
Teknova 0.01M PBS with 0.02% Tween-80. 1000mL, Sterile
0.85% Sodium Chloride, 0.12% Sodium Phosphate Dibasic, 0.022% Sodium Phosphate Monobasic, 0.02% Tween-80
LI COR BIOTECH LLC Intercept (TBS) Protein-Free Blocking Buffer (500 mL)
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in TBS and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Research Products International Corp BSA-Tween 20 Blocking Solution in TBS, 500 Milliliters
Contains 3% BSA and 0.05% Tween 20 in TBS buffer. Optimized for use in western blotting to minimize non-specific binding of antibodies.
Research Products International Corp Casein Blocking Solution in TBS, 1 Liter
Contains 3% Casein in TBS buffer. Optimized for use in western blotting to minimize non-specific binding of antibodies.
Seracare Life Sciences Inc 10X DULBECCO S PBS W/ TWEEN 20
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. pH=7.4. Conductivity controlled.
Seracare Life Sciences Inc 10% BSA DILUENT/BLOCKING SOL
Convenient antibody diluent/blocking solution concentrate containing 10% bovine serum albumin in phosphate buffer. Compatible with most assay formats.
Seracare Life Sciences Inc 10X TBS W/ 10% TWEEN 20(TBST)
10X Tris-buffered Saline (TBS) with 10% Tween 20 is used as a common Western blot wash solution and general wash buffer in immunoassay applications. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. This formulation of TBST has a higher concentration of Tween 20 and provides a more stringent wash than standard TBST formulations.
Seracare Life Sciences Inc 10X DULBECCO S PBS W/ TWEEN 20
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. pH=7.4. Conductivity controlled.
Biocare Medical, LLC Mouse-on-Mouse HRP-Polymer Bundle - 6.0 ml
The Mouse-on-Mouse HRP-Polymer is specially designed for using mouse primary antibodies on mouse tissues with minimum cross-reactivity to endogenous mouse IgG. Utilization of this goat anti-mouse horseradish peroxidase (HRP) polymer is advantageous over traditional biotinylation methods. Biotinylated anti-mouse secondary antibodies can bind to endogenous mouse IgG in the tissue in addition to the primary antibody, leading to troublesome background staining. Biocare Medical's biotin-free polymer technology provides the elimination of endogenous biotin, reduction of IHC steps and increased sensitivity.
Electron Microscopy Sciences Aurion Blocking Solution (Protein A & G) 30 ML
Aurion has developed Blocking Solutions which effectively block ‘sticky' surfaces by multi-point hydrophobic and charge-based interactions. The Blocking Solutions are specifically designed to meet the characteristics of each type of secondary conjugate. With adequate blocking and incubation media, background reactions are minimized whereas specific reactions are not hampered.